Agro-Waste-Mediated Green Synthesis of Silver Nanoparticles from Harum Manis and Sala Mango Peel Waste and Their Antimicrobial Activity

Fadhilatun Nisa Hamzah; Zainab Razali; Nur Syafiqah Rahim; Muhammad Azhar Zulkffle; Roejhan Md Kawi

doi:10.56532/mjsat.v6iS1.787

Authors

Fadhilatun Nisa Hamzah Faculty of Applied Sciences, Universiti Teknologi MARA, Perlis Branch, Arau Campus, 02600 Arau, Perlis
Zainab Razali Faculty of Applied Sciences, Universiti Teknologi MARA, Perlis Branch, Arau Campus, 02600 Arau, Perlis https://orcid.org/0000-0002-1592-4084
Nur Syafiqah Rahim Faculty of Applied Sciences, Universiti Teknologi MARA, Perlis Branch, Arau Campus, 02600 Arau, Perlis
Muhammad Azhar Zulkffle Faculty of Applied Sciences, Universiti Teknologi MARA, Perlis Branch, Arau Campus, 02600 Arau, Perlis https://orcid.org/0000-0003-2378-1009
Roejhan Md Kawi Faculty of Electrical Engineering & Technology, Universiti Malaysia Perlis, Pauh Putra Campus, 02600 Arau, Perlis, Malaysia

DOI:

https://doi.org/10.56532/mjsat.v6iS1.787

Keywords:

Green Synthesis, Agro-waste Utilisation, Mango Peel Extract, Silver nanoparticles, Antimicrobial activity

Abstract

Green synthesis of silver nanoparticles (AgNPs) is increasingly recognised as an environmentally sustainable alternative to traditional physicochemical synthesis methods. The growing challenge of antimicrobial resistance has further intensified the search for effective and sustainable antimicrobial materials. In this study, mango peel agro-waste from the Harum Manis and Sala varieties was utilised as a natural reducing and stabilising agent for the biosynthesis of AgNPs.The formation of AgNPs was demonstrated using UV–Visible spectroscopy, which showed characteristic surface plasmon resonance peaks within the range of 400–500 nm. Fourier Transform Infrared (FTIR) analysis revealed the presence of functional groups associated with biomolecules responsible for nanoparticle reduction and stabilisation. The antimicrobial activity of the synthesised AgNPs was assessed against Escherichia coli and Staphylococcus aureus using disc diffusion and minimum inhibitory concentration (MIC) assays. Both mango peel-derived AgNPs exhibited moderate antibacterial activity with inhibition zones ranging from 8–10 mm, while the MIC value for both bacterial strains was determined to be 0.1 g/mL. These findings demonstrate the potential of mango peel agro-waste as a renewable resource for AgNP synthesis and highlight its promising application as an eco-friendly antimicrobial agent.

References

Khan, I., Saeed, K., & Khan, I. (2019). Nanoparticles: Properties, Applications and Toxicities. Arabian Journal of Chemistry 12(7), 908–931. doi: https://doi.org/10.1016/j.arabjc.2017.05.011

Bruna, T., Maldonado-Bravo, F., Jara, P., & Caro, N. (2021). Silver Nanoparticles and Their Antibacterial Applications. International Journal of Molecular Sciences 22(13), 7202. doi: https://doi.org/10.3390/ijms22137202

Razali, Z, Jami'an, N.S.A, Sidik N.J. & Kawi, R.M (2025). Elucidation Study of Bioavailable Potential Among Plant-based Silver Nanoparticles Fabricated from Several Local Fruit Peels. Scientific Research Journal, 22(1), 223-241doi: https://doi.org/10.24191/srj.v22i1.17045

Mancuso, G., Midiri, A., Gerace, E., & Biondo, C. (2021). Bacterial Antibiotic Resistance: The Most Critical Pathogens. Pathogens 10(10). 1310. doi: https://doi.org/10.3390/pathogens10101310

Rajeshkumar, S., Parameswari, R. P., Sandhiya, D., Al-Ghanim, K. A., Nicoletti, M., & Govindarajan, M. (2023). Green Synthesis, Characterization and Bioactivity of Mangifera indica Seed-Wrapped Zinc Oxide Nanoparticles. Molecules, 28(6), 2818. doi: https://doi.org/10.3390/molecules28062818

Veerasamy, R., Xin, T. Z., Gunasagaran, S., Xiang, T. F. W., Yang, E. F. C., Jeyakumar, N., & Dhanaraj, S. A. (2011). Biosynthesis of Silver Nanoparticles using Mangosteen Leaf Extract and Evaluation of Their Antimicrobial Activities. Journal of Saudi Chemical Society, 15(2), 113–120. doi: https://doi.org/10.1016/j.jscs.2010.06.004

Yahya, S. M., Abdulmumin, Y., Abdulmumin, T. M., Sagagi, B. S., Murtala, M., Salau, A. K., & Hassan, S. A. (2021). Biological synthesis, characterization and antimicrobial effect of silver nanoparticles (Ag-NPs) using aqueous extract of mango pulp (Mangifera indica). Journal of Complementary and Alternative Medical Research, 13(4), 39-50. doi: https://doi.org/10.9734/jocamr/2021/v13i430233

Razali, Z., Md Kawi, R., & Jaafar Sidik, N. (2022). Impact of temperature and pH on antioxidant activity of green silver nanoparticles fabricated from Ananas comosus peel extracts. IOP Conference Series: Earth and Environmental Science, 1019(1), 012006. doi: https://doi.org/10.1088/1755-1315/1019/1/012006

Angamuthu, S., Thangaswamy, S., Raju, A., Husain, F. M., Ahmed, B., Al-Shabib, N. A., Hakeem, M. J., Shahzad, S. A., Abudujayn, S. A., & Alomar, S. Y. (2023). Biogenic Preparation and Characterization of Silver Nanoparticles from Seed Kernel of Mangifera indica and Their Antibacterial Potential against Shigella spp. Molecules, 28(6). doi: https://doi.org/10.3390/molecules28062468

Flieger, J., Franus, W., Panek, R., Szymańska-Chargot, M., Flieger, W., Flieger, M., & Kołodziej, P. (2021). Green Synthesis of Silver Nanoparticles Using Natural Extracts with Proven Antioxidant Activity. Molecules, 26(16). doi: https://doi.org/10.3390/molecules26164986

Nandiyanto, A. B. D., Oktiani, R., & Ragadhita, R. (2019). How to read and interpret FTIR spectroscope of organic material. Indonesian Journal of Science and Technology, 4(1), 97–118. doi: https://doi.org/10.17509/ijost.v4i1.15806

Ahmed, R. H., & Mustafa, D. E. (2020). Green Synthesis of Silver Nanoparticles Mediated by Traditionally used Medicinal Plants in Sudan. International Nano Letters 10(1). 1-14 doi: https://doi.org/10.1007/s40089-019-00291-9

John, S., Lydia, E., Iyer, P., Monica, S. J., & Thambi, P. A. (2016). Antimicrobial Efficacy of Mango Peel Powder and Formulation of Recipes using Mango Peel Powder (Mangifera indica L.). International Journal of Home Science, 2(2), 155–161. www.homesciencejournal.com

Razali, Z., Kawi, R. M., Sidik, N. J., Mohamad, S., Sarizan, N. M., Asman, N. A., & Ahmad Kamil, K. (2025). Mutual Bilateral Antibacterial and Toxicity Potentialities of Silver Nanoparticles Biosynthesized from Toxic Dioscorea Hispida. Journal of Advanced Research in Micro and Nano Engineering, 16(1), 1–11 doi: https://doi.org/10.37934/armne.35.1.110

Wu, Y., Yang, Y., Zhang, Z., Wang, Z., Zhao, Y., & Sun, L. (2018). A Facile Method to Prepare Size-tunable Silver Nanoparticles and Its Antibacterial Mechanism. Advanced Powder Technology, 29(2), 407–415. doi: https://doi.org/10.1016/j.apt.2017.11.028

Garibo, D., Borbón-Nuñez, H. A., de León, J. N. D., García Mendoza, E., Estrada, I., Toledano-Magaña, Y., Tiznado, H., Ovalle-Marroquin, M., Soto-Ramos, A. G., Blanco, A., Rodríguez, J. A., Romo, O. A., Chávez-Almazán, L. A., & Susarrey-Arce, A. (2020). Green Synthesis of Silver Nanoparticles using Lysiloma acapulcensis Exhibit High-antimicrobial Activity. Scientific Reports, 10(1), 12805. doi: https://doi.org/10.1038/s41598-020-69606-7

Burange, P. J., Tawar, M. G., Bairagi, R. A., Malviya, V. R., Sahu, V. K., Shewatkar, S. N., Sawarkar, R. A., & Mamurkar, R. R. (2021). Synthesis of Silver Nanoparticles by using Aloe vera and Thuja orientalis Leaves Extract and Their Biological Activity: A Comprehensive Review. Bulletin of the National Research Centre, 45(1), 1-13. doi: https://doi.org/10.1186/s42269-021-00639-2